Mapping of the human CENP-B gene to chromosome 20 and the CENP-C gene to chromosome 12 by a rapid cycle DNA amplification procedure

K Sugimoto; H Yata; M Himeno

doi:10.1006/geno.1993.1312

Mapping of the human CENP-B gene to chromosome 20 and the CENP-C gene to chromosome 12 by a rapid cycle DNA amplification procedure

Genomics. 1993 Jul;17(1):240-2. doi: 10.1006/geno.1993.1312.

Authors

K Sugimoto¹, H Yata, M Himeno

Affiliation

¹ Department of Agricultural Chemistry, University of Osaka Prefecture, Japan.

PMID: 8406460
DOI: 10.1006/geno.1993.1312

Abstract

By optimizing the primer-annealing temperature in a rapid air cycling procedure, two human DNA sequences encoding centromere proteins B and C (CENP-B and CENP-C) were specifically amplified without any detectable amplification of highly homologous rodent DNA sequences. Using a panel of rodent/human hybrid DNA, the genes for human CENP-B and CENP-C were conveniently mapped to chromosomes 20 and 12, respectively.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Autoantigens*
Base Sequence
Centromere Protein B
Chromosomal Proteins, Non-Histone / genetics*
Chromosome Mapping
Chromosomes, Human, Pair 12*
Chromosomes, Human, Pair 20*
Cricetinae
DNA-Binding Proteins*
Genes*
Humans
Hybrid Cells
Molecular Sequence Data
Polymerase Chain Reaction / methods*

Substances

Autoantigens
CENPB protein, human
Centromere Protein B
Chromosomal Proteins, Non-Histone
DNA-Binding Proteins
centromere protein C