Crystallization and preliminary crystallographic characterization of the copper-containing amine oxidase from pea seedlings

J Mol Biol. 1993 Jan 5;229(1):243-5. doi: 10.1006/jmbi.1993.1022.

Abstract

The copper-containing amine oxidase from pea seedlings has been crystallized using lithium sulfate as precipitant at pH 5.2. The unit cell is orthorhombic, space group P2(1)2(1)2(1), with dimensions a = 89.3 A, b = 113.4 A, c = 199.0 A. The mass of the asymmetric unit is 131(+/- 13) kDa, consistent with independent evidence that the molecule has two approximately 66 kDa subunits. The crystals diffract to 2.5 A in a synchrotron X-ray beam.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amine Oxidase (Copper-Containing) / chemistry*
  • Crystallization
  • Fabaceae / enzymology*
  • Plants, Medicinal*
  • X-Ray Diffraction

Substances

  • Amine Oxidase (Copper-Containing)