Amnion fluid samples were obtained from 100 pregnant women in the last week of pregnancy just before in labor. In detecting of antibacterial effectiveness, S. aureus ATCC 29213, E. coli ATCC 25922 and S. agalactiae ATCC 13813 strains were used. Antibacterial effectiveness of amnion fluid to these bacteria are found respectively as; 74%, 63% and 73.9%.