In this study, HBsAg, Anti-HBc and Anti-HBs have been investigated in 172 prostitutes and 50 healthy women with ELISA. Anti-HBc seropositivity was increasing with age in prostitutes. No significant difference was found between Hepatitis-B marker seropositivity and working period of prostitutes. In prostitutes, 6.3% HBsAg, 62.7% Anti-HBc, 50.5% Anti-HBs seropositivity and 69.1% total seropositivity was found. Statistically, except HBsAg seropositivity, seropositivity in prostitutes was higher than the control group. Therefore, prostitutes are an important risk group for Hepatitis-B infection.