Abstract
A cDNA encoding rat intestinal 15 kDa protein was isolated and sequenced from a rat ileum-specific cDNA library. This cDNA was found to contain an open reading frame of 384 nucleotides as well as 5' (27 nucleotides) and 3' (46 nucleotides) non-coding regions. The deduced sequence of 127 amino acids was identical to that of rat I-15P which was purified from rat intestinal epithelium. The nucleotide sequence of the open reading frame exhibited 79% identity to that of the porcine gastrotropin. Northern blot analysis indicated that the same size of transcript as that of the ileum was detected in the ovary, suggesting that I-15P or a homologous protein might be involved in the metabolism of steroids in steroid hormone-producing tissues.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Carrier Proteins / genetics*
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Cloning, Molecular / methods
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DNA / genetics*
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Fatty Acid-Binding Protein 7
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Fatty Acid-Binding Proteins
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Female
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Gastrointestinal Hormones / genetics
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Gene Library
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Ileum / metabolism*
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Male
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Molecular Sequence Data
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Molecular Weight
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Muscle, Smooth / metabolism*
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Neoplasm Proteins*
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Nerve Tissue Proteins*
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Open Reading Frames
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Organ Specificity
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Ovary / metabolism
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Polymerase Chain Reaction / methods
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Rats
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Rats, Sprague-Dawley
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Sequence Homology, Nucleic Acid
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Swine
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Transcription, Genetic
Substances
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Carrier Proteins
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Fabp7 protein, rat
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Fatty Acid-Binding Protein 7
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Fatty Acid-Binding Proteins
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Gastrointestinal Hormones
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Neoplasm Proteins
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Nerve Tissue Proteins
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fatty acid-binding protein 6
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DNA
Associated data
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GENBANK/S52878
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GENBANK/S55582
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GENBANK/S64629
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GENBANK/S64630
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GENBANK/S64631
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GENBANK/S64632
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GENBANK/S64635
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GENBANK/S64636
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GENBANK/S64637
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GENBANK/U07167