Localization of the human oncostatin M gene (OSM) to chromosome 22q12, distal to the Ewing's sarcoma breakpoint

Cytogenet Cell Genet. 1993;62(1):32-4. doi: 10.1159/000133439.

Abstract

Using fluorescence in situ hybridization, a cosmid clone containing the gene for oncostatin M (OSM) was mapped to human chromosome 22q12, placing the OSM gene in the same chromosome band as the leukemia-inhibitory factor gene (LIF). The location of the OSM gene was determined relative to the t(11;22)(q24;q12) of Ewing's sarcoma and found to be distal to the translocation breakpoint on chromosome 22. Analysis of physical distances by pulsed-field gel electrophoresis demonstrated further that the two genes lie within 500 kb of each other.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Chromosomes, Human, Pair 11
  • Chromosomes, Human, Pair 22*
  • DNA, Single-Stranded
  • Humans
  • In Situ Hybridization, Fluorescence
  • Molecular Sequence Data
  • Oncostatin M
  • Peptides / genetics*
  • Sarcoma, Ewing / genetics*
  • Translocation, Genetic
  • Tumor Cells, Cultured

Substances

  • DNA, Single-Stranded
  • OSM protein, human
  • Peptides
  • Oncostatin M