Abstract
A genomic DNA clone encoding cytosolic ascorbate peroxidase was isolated from a genomic library of Arabidopsis thaliana, using a cDNA for the enzyme as a probe. Nucleotide sequence and primer extension analyses of this gene (APX1) revealed nine exons split by eight introns, one of which is inserted in the 5'-untranslated region. The exon/intron organization of the APX1 gene differs from that of the guaiacol peroxidase genes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Arabidopsis / enzymology*
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Ascorbate Peroxidases
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Base Sequence
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Blotting, Western
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Cytosol / enzymology*
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DNA*
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Exons
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Genes, Plant*
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Genomic Library
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Introns
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Molecular Sequence Data
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Peroxidases / genetics*
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Restriction Mapping
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Sequence Homology, Amino Acid
Substances
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DNA
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Peroxidases
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Ascorbate Peroxidases
Associated data
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GENBANK/D14442
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GENBANK/X68456
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GENBANK/X68457
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GENBANK/X68458
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GENBANK/X68459
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GENBANK/X68460
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GENBANK/X68461
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GENBANK/X68462
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GENBANK/X68463
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GENBANK/X70141