The local anti-inflammatory action of dexamethasone in the rat carrageenin oedema model is reversed by an antiserum to lipocortin 1

Br J Pharmacol. 1993 Jan;108(1):62-5. doi: 10.1111/j.1476-5381.1993.tb13440.x.

Abstract

1. A local pre-injection of 1 micrograms dexamethasone sodium phosphate strongly inhibited (> 60% inhibition at 3 h; P < 0.001 at all time points) the development of carrageenin-induced paw oedema in the rat induced by a subplantar injection of 0.1 ml, 2% carrageenin. 2. Coinjection of a polyclonal rabbit antiserum raised against human 1-188 recombinant lipocortin 1, which also recognised the rat protein, reversed the inhibitory action of dexamethasone (P < 0.05 at 4 h and 5 h). At the highest volume used (40 microliters) control antisera were without any effect. 3. These data further support the concept that lipocortin 1 is involved in the anti-inflammatory mechanism of action of the glucocorticoids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Annexin A1 / immunology
  • Annexin A1 / physiology*
  • Blotting, Western
  • Dexamethasone / administration & dosage
  • Dexamethasone / pharmacology*
  • Dexamethasone / therapeutic use
  • Edema / chemically induced
  • Edema / drug therapy*
  • Electrophoresis, Polyacrylamide Gel
  • Immune Sera / administration & dosage
  • Immune Sera / immunology
  • Male
  • Rabbits
  • Rats
  • Rats, Wistar

Substances

  • Annexin A1
  • Immune Sera
  • Dexamethasone