An alphoid DNA sequence conserved in all human and great ape chromosomes: evidence for ancient centromeric sequences at human chromosomal regions 2q21 and 9q13

Hum Genet. 1993 Feb;90(6):577-83. doi: 10.1007/BF00202474.

Abstract

Using vector-CENP-B box polymerase chain reaction (PCR) we isolated and cloned from a human chromosome 21-specific plasmid library, a 1 kb DNA sequence, named p alpha H21. In in situ hybridization experiments, p alpha H21 hybridized, under high stringency conditions, to the centromeric region of all the human, chimpanzee, gorilla and orangutan chromosomes. On human chromosomes p alpha H21 also identified non-centromeric sequences at 2q21 (locus D2F33S1) and 9q13 (locus D9F33S2). The possible derivation of these sequences from ancestral centromeres is discussed. Sequence analysis confirmed the alphoid nature of the whole p alpha H21 insert.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Southern
  • Centromere*
  • Chromosomes, Human, Pair 2*
  • Chromosomes, Human, Pair 9*
  • Conserved Sequence*
  • DNA, Satellite / analysis
  • Female
  • Gene Library
  • Gorilla gorilla / genetics
  • Hominidae / genetics*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Molecular Sequence Data
  • Pan troglodytes / genetics
  • Polymerase Chain Reaction
  • Pongo pygmaeus / genetics
  • Repetitive Sequences, Nucleic Acid
  • Sequence Analysis, DNA

Substances

  • DNA, Satellite

Associated data

  • GENBANK/L13238
  • GENBANK/L13296
  • GENBANK/L13297
  • GENBANK/L14001
  • GENBANK/L14002
  • GENBANK/L14003
  • GENBANK/M64321
  • GENBANK/S70572
  • GENBANK/S70573
  • GENBANK/X66424