Production of monoclonal antibodies against Clostridium difficile cytotoxin using immunosorbent binding bioassay procedure

C Depitre; R M L'Haridon; D M Lyerly; L A Barroso; T D Wilkins; A Andremont; G Corthier

doi:10.1016/0041-0101(93)90284-p

Production of monoclonal antibodies against Clostridium difficile cytotoxin using immunosorbent binding bioassay procedure

Toxicon. 1993 Feb;31(2):181-6. doi: 10.1016/0041-0101(93)90284-p.

Authors

C Depitre¹, R M L'Haridon, D M Lyerly, L A Barroso, T D Wilkins, A Andremont, G Corthier

Affiliation

¹ Unité d'Ecologie et de Physiologie du Système Digestif, Centre de Recherches de Jouy, Jouy-en-Josas, France.

PMID: 8456445
DOI: 10.1016/0041-0101(93)90284-p

Abstract

In the following study, a novel screening approach was used to develop monoclonal antibodies specific for toxin B of Clostridium difficile. The approach, which consisted of an immunosorbent binding bioassay (ISBBA), is based on antigen immunocapture by monoclonal antibodies and detection of biological activity. Our results showed ISBBA, which uses unpurified antigen, to be more sensitive than the neutralization assay and ELISA for the detection of toxin B antibody.

MeSH terms

Animals
Antibodies, Monoclonal / analysis
Antibodies, Monoclonal / biosynthesis*
Ascites / immunology
Bacterial Proteins*
Bacterial Toxins / immunology*
Blotting, Northern
Clostridioides difficile / metabolism
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Hybridomas / immunology
Immunosorbent Techniques
Mice
Neutralization Tests
Rats
Spleen / cytology

Substances

Antibodies, Monoclonal
Bacterial Proteins
Bacterial Toxins
toxB protein, Clostridium difficile