Laser-light induced chromosome aberrations in Chinese hamster cells

Photochem Photobiol. 1993 Mar;57(3):577-9. doi: 10.1111/j.1751-1097.1993.tb02336.x.

Abstract

Wild-type Chinese hamster cells CHO K1 and their radiosensitive mutant xrs5 were irradiated at 308 nm, using light pulses of a XeCl excimer laser with total energy fluences of 0.1 kJ/m2 to 4.08 kJ/m2. Chromosome-type and chromatid-type chromosome aberrations have been observed at pulse irradiances of 2.5 x 10(7) W/m2 and 1.7 x 10(8) W/m2, indicating that in mammalian cells DNA double-strand breaks occur already in this irradiance range. The results obtained with laser irradiation are compared with X-ray irradiation.

MeSH terms

  • Animals
  • CHO Cells
  • Chromosome Aberrations*
  • Chromosomes / radiation effects*
  • Cricetinae
  • Dose-Response Relationship, Radiation
  • Lasers
  • Light