Abstract
Fumarase C purified from Escherichia coli has been crystallized in the presence of polyethylene glycol in both a citrate buffer at pH 5.3 and a 3-(4-morpholino)-propanesulfonic acid buffer at pH 7.5 yielding two crystal forms. An orthorhombic C222(1) form was obtained in citrate at pH 5.3 and an orthorhombic I222 form was obtained in 3-(4-morpholino)-propanesulfonic acid (pH 7.5). Complete native data sets have been collected on both crystal forms: the C222(1) form is complete to 2.10 A and the I222 form is complete to 2.20 A.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Amino Acid Sequence
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Chromatography, Affinity
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Chromatography, Ion Exchange
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Crystallization
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Fumarate Hydratase / chemistry*
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Fumarate Hydratase / genetics
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Fumarate Hydratase / isolation & purification
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Genes, Bacterial
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Isoenzymes / chemistry*
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Isoenzymes / genetics
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Isoenzymes / isolation & purification
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Kinetics
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Molecular Sequence Data
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Protein Conformation
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X-Ray Diffraction
Substances
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Isoenzymes
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Fumarate Hydratase