The complete sequences of plasmids pFNeo and pMH-Neo: convenient expression vectors for high-level expression of eukaryotic genes in hematopoietic cell lines

Gene. 1993 May 30;127(2):267-8. doi: 10.1016/0378-1119(93)90731-h.

Abstract

The eukaryotic expression vector, pFNeo, confers resistance to the antibiotic G418 and directs hematopoietic-specific expression of proteins under the control of the long terminal repeat from the Friend spleen focus-forming virus. Here, we report the entire nucleotide sequence of pFNeo. We have also constructed and sequenced a pFNeo-based expression vector (pMH-Neo) that carries an improved multiple cloning site region for easier subcloning. The utility of both vectors was demonstrated by transfection of murine T-cell hybridomas by electroporation. Transfection with either pFNeo or pMH-Neo yielded a high frequency (1 in 2 x 10(4)) of G418-resistant cell lines.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Base Sequence
  • Cell Line
  • Cloning, Molecular / methods*
  • Friend murine leukemia virus / genetics
  • Gene Expression
  • Genetic Vectors
  • Gentamicins / pharmacology
  • Hematopoiesis
  • Humans
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Plasmids*
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Spleen Focus-Forming Viruses / genetics
  • Transfection / methods*

Substances

  • Anti-Bacterial Agents
  • Gentamicins
  • Oligodeoxyribonucleotides
  • antibiotic G 418

Associated data

  • GENBANK/L03653
  • GENBANK/L03654
  • GENBANK/L07040
  • GENBANK/L07041
  • GENBANK/L07632
  • GENBANK/X54209
  • GENBANK/X67280
  • GENBANK/X67281
  • GENBANK/X67282
  • GENBANK/X67283