Linkage mapping detects two secondary microdeletions in cell hybrid HHW1064, used to isolate DNA probes from within 5q11.2-->q13.3

L E Bernard; J D McPherson; J J Wasmuth; C N Kreklywich; L M Brzustowicz; S Wood

doi:10.1159/000133558

Linkage mapping detects two secondary microdeletions in cell hybrid HHW1064, used to isolate DNA probes from within 5q11.2-->q13.3

Cytogenet Cell Genet. 1993;64(1):46-8. doi: 10.1159/000133558.

Authors

L E Bernard¹, J D McPherson, J J Wasmuth, C N Kreklywich, L M Brzustowicz, S Wood

Affiliation

¹ Department of Medical Genetics, University of British Columbia, Vancouver, Canada.

PMID: 8508678
DOI: 10.1159/000133558

Abstract

The somatic cell hybrid HHW1064 contains a single human chromosome 5 with an interstitial deletion for 5q11.2-->q13.3. Twenty human clones were isolated by Alu-PCR differential hybridization. Mapping these clones indicates that HHW1064 contains two additional secondary microdeletions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Mapping
Chromosomes, Human, Pair 5*
Female
Gene Deletion*
Genetic Linkage
Genetic Markers
Humans
Hybrid Cells
Karyotyping
Male
Polymerase Chain Reaction

Substances

Genetic Markers