Errors in X-ray beam alignment account for many of the exposure errors in bitewing radiography. A new method for measuring these alignment errors is described. The alignment accuracy of two different models of inexpensive plastic positioning devices, used for bitewing radiography, were quantified and compared. The technique involves documenting the alignment of an 'ideal' reference radiograph and successive exposures. It permits calculation of X-ray beam-film alignment errors in all subsequent radiographs using the 'ideal' reference. Bitewing radiographs were taken on 61 patients and 19 of these were re-radiographed at 6-month intervals over a 2-year period, providing a total of 156 radiographs for the study. Horizontal and vertical angular deviations were measured and total alignment errors calculated for each radiograph. The average angular alignment error was less than 2 degrees total angular error (1.3-2.4 degrees, 95% confidence intervals). It is concluded that these devices can be used when X-ray beam-film alignment error below 2.5 degrees is acceptable.