Molecular cloning and sequencing of a human somatostatin receptor, hSSTR4

Biochem Biophys Res Commun. 1993 Jun 15;193(2):648-52. doi: 10.1006/bbrc.1993.1673.

Abstract

We recently reported the cloning of a somatostatin receptor (SSTR) subtype from a rat genomic library designated rSSTR4. In the present study, we report the cloning of the human SSTR4 gene. A human genomic library was screened with a 1.2 kb fragment of rSSTR4 containing the full open reading frame and a genomic clone, hSSTR4, was isolated. The deduced amino acid sequence of this clone encoded a protein of 388 amino acids and is a member of the G-protein coupled receptor superfamily. Comparison of rat and human SSTR4 sequences demonstrated 89% identity. In addition, the sequence of hSSTR4 shows 61%, 46%, and 47% sequence identity with previously identified isoforms hSSTR1, hSSTR2, and hSSTR3, respectively.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Membrane / metabolism
  • Cloning, Molecular / methods
  • DNA
  • Genomic Library
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Rats
  • Receptors, Somatostatin / biosynthesis*
  • Receptors, Somatostatin / genetics*
  • Sequence Homology, Amino Acid

Substances

  • Receptors, Somatostatin
  • DNA

Associated data

  • GENBANK/L14856
  • GENBANK/U11079
  • GENBANK/U11080
  • GENBANK/U11081
  • GENBANK/U11082
  • GENBANK/U11083
  • GENBANK/U11084
  • GENBANK/U11085
  • GENBANK/U11086
  • GENBANK/U11087