Signal processing in slit-scan flow cytometry of cell conjugates

Cytometry. 1993;14(4):459-64. doi: 10.1002/cyto.990140417.

Abstract

The design and implementation of a real-time signal processing system for slit-scan flow cytometry is described. The system is used to measure the separate scatter and fluorescence peak heights of 2 adherent cells. Preliminary measurements of changes in the membrane potential induced by interactions between natural killer (NK) cells and their target cells are presented.

MeSH terms

  • Barbiturates
  • Cell Adhesion*
  • Cytotoxicity, Immunologic
  • Electronics / instrumentation
  • Flow Cytometry / instrumentation*
  • Fluorescence
  • Humans
  • Isoxazoles
  • Killer Cells, Natural / cytology*
  • Leukemia, Erythroblastic, Acute
  • Membrane Potentials
  • Scattering, Radiation
  • Tumor Cells, Cultured

Substances

  • Barbiturates
  • Isoxazoles
  • bis(1,3-dibutylbarbiturate)trimethine oxonol