High-level production of biologically active human cytosolic phospholipase A2 in baculovirus-infected insect cells

B Y Amegadzie; D Jiampetti; R J Craig; E Appelbaum; A R Shatzman; R J Mayer; A G DiLella

doi:10.1016/0378-1119(93)90580-v

High-level production of biologically active human cytosolic phospholipase A2 in baculovirus-infected insect cells

Gene. 1993 Jun 30;128(2):307-8. doi: 10.1016/0378-1119(93)90580-v.

Authors

B Y Amegadzie¹, D Jiampetti, R J Craig, E Appelbaum, A R Shatzman, R J Mayer, A G DiLella

Affiliation

¹ Department of Gene Expression Sciences, SmithKline Beecham Pharmaceuticals, King of Prussia, PA 19406-0939.

PMID: 8514199
DOI: 10.1016/0378-1119(93)90580-v

Abstract

Infection of Spodoptera frugiperda insect cells with a recombinant baculovirus expressing human cytosolic phospholipase A2 (cPLA2) resulted in the production of biologically active protein. The level of recombinant human cPLA2 production in infected insect cells was at least 50-fold higher than that observed in human monoblast U937 cells.

MeSH terms

Animals
Baculoviridae
Cytosol / enzymology
DNA, Recombinant
Humans
Moths
Phospholipases A / biosynthesis
Phospholipases A / genetics*
Phospholipases A2
Recombinant Fusion Proteins / biosynthesis
Recombinant Fusion Proteins / genetics

Substances

DNA, Recombinant
Recombinant Fusion Proteins
Phospholipases A
Phospholipases A2