Abstract
Sodium butyrate (NaBu) but not dimethylsulfoxide (DMSO) induced the synthesis of villin, a protein of the brush border microvilli cytoskeleton, in a rat colon cancer cell line. Neither NaBu nor DMSO altered mdr 1-mRNA expression or multidrug resistance (MDR)--associated cellular transport of doxorubicin. These results show that mdr 1 gene expression and activity are independent of other brush border proteins induced by differentiating agents at the apical pole of the epithelial cell.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Biological Transport, Active / drug effects
-
Butyrates / pharmacology*
-
Butyric Acid
-
Carrier Proteins / biosynthesis*
-
Cell Differentiation / drug effects
-
Cell Differentiation / physiology
-
Cell Membrane / metabolism
-
Colonic Neoplasms / genetics*
-
Colonic Neoplasms / metabolism*
-
Colonic Neoplasms / pathology
-
Dimethyl Sulfoxide / pharmacology
-
Doxorubicin / pharmacokinetics
-
Drug Resistance / genetics*
-
Gene Expression / genetics*
-
Membrane Glycoproteins / metabolism
-
Membrane Glycoproteins / physiology
-
Microfilament Proteins / biosynthesis*
-
Rats
-
Rats, Inbred Strains
-
Tumor Cells, Cultured / drug effects
Substances
-
Butyrates
-
Carrier Proteins
-
Membrane Glycoproteins
-
Microfilament Proteins
-
villin
-
Butyric Acid
-
Doxorubicin
-
Dimethyl Sulfoxide