Abstract
The switch from short- to long-term facilitation induced by behavioral sensitization in Aplysia involves CREB-like proteins, as well as the immediate-early gene ApC/EBP. Using the bZIP domain of ApC/EBP in a two-hybrid system, we have cloned ApCREB2, a transcription factor constitutively expressed in sensory neurons that resembles human CREB2 and mouse ATF4. ApCREB2 represses ApCREB1-mediated transcription in F9 cells. Injection of anti-ApCREB2 antibodies into Aplysia sensory neurons causes a single pulse of serotonin (5-HT), which induces only short-term facilitation lasting minutes, to evoke facilitation lasting more than 1 day. This facilitation has the properties of long-term facilitation: it requires transcription and translation, induces the growth of new synaptic connections, and occludes further facilitation by five pulses of 5-HT.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Activating Transcription Factor 2
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Amino Acid Sequence
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Animals
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Aplysia
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Base Sequence
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CCAAT-Enhancer-Binding Proteins
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Cells, Cultured
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Cyclic AMP Response Element-Binding Protein / physiology
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DNA / metabolism
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DNA-Binding Proteins / metabolism
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Humans
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Long-Term Potentiation / physiology*
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Memory / physiology*
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Mice
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Molecular Sequence Data
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Nerve Tissue Proteins*
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Neurons, Afferent / physiology*
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Nuclear Proteins / metabolism
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Organ Specificity
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Phosphorylation
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RNA, Messenger / analysis
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Repressor Proteins / biosynthesis
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Repressor Proteins / genetics
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Repressor Proteins / metabolism
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Repressor Proteins / physiology*
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Sequence Homology, Amino Acid
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Serotonin / pharmacology
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Synapses / physiology
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Trans-Activators / physiology
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Transcription Factors / analysis
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Transcription Factors / biosynthesis
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Transcription Factors / genetics
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Transcription Factors / physiology*
Substances
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Activating Transcription Factor 2
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ApCREB2 protein, Aplysia californica
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CCAAT-Enhancer-Binding Proteins
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Cyclic AMP Response Element-Binding Protein
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DNA-Binding Proteins
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Nerve Tissue Proteins
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Nuclear Proteins
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RNA, Messenger
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Repressor Proteins
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Trans-Activators
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Transcription Factors
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Serotonin
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DNA