Extracellular domain of type I receptor for transforming growth factor-beta: molecular modelling using protectin (CD59) as a template

FEBS Lett. 1995 Nov 27;376(1-2):31-6. doi: 10.1016/0014-5793(95)01239-7.

Abstract

We have observed that the extracellular domain of T beta RI and protectin (CD59), an inhibitor of the membrane attack complex of complement, share structural features, a distinct spacing of ten cysteines and a C-terminal 'Cys-box'. Based on these common features and the recently determined NMR-structure of protectin, a three-dimensional model for the extracellular domain of T beta RI was constructed. After energy minimization and molecular dynamics simulation, a structure with four extending fingers (pes quattvordigitorum) and two clusters of charged residues was obtained. This model provides a view to the understanding of interactions between T beta RI, T beta RII and TGF beta during ligand recognition and signal transduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • CD59 Antigens / chemistry*
  • Complement Membrane Attack Complex / antagonists & inhibitors
  • Computer Graphics
  • Cysteine / chemistry
  • Models, Molecular
  • Molecular Sequence Data
  • Protein Structure, Tertiary
  • Receptors, Transforming Growth Factor beta / chemistry*
  • Sequence Alignment

Substances

  • CD59 Antigens
  • Complement Membrane Attack Complex
  • Receptors, Transforming Growth Factor beta
  • Cysteine