Crystallization and preliminary X-ray diffraction studies of complexes between an influenza hemagglutinin and Fab fragments of two different monoclonal antibodies

B Gigant; D Fleury; T Bizebard; J J Skehel; M Knossow

doi:10.1002/prot.340230113

Crystallization and preliminary X-ray diffraction studies of complexes between an influenza hemagglutinin and Fab fragments of two different monoclonal antibodies

Proteins. 1995 Sep;23(1):115-7. doi: 10.1002/prot.340230113.

Authors

B Gigant¹, D Fleury, T Bizebard, J J Skehel, M Knossow

Affiliation

¹ Laboratoire de Biologie Structurale, UMR 9920, CNRS-Université Paris-Sud, C.N.R.S. 91198, Gif-sur-Yvette, France.

PMID: 8539243
DOI: 10.1002/prot.340230113

Abstract

Fab fragments from two different monoclonal antibodies (BH151 and HC45) which bind to the same antigenic region of the influenza hemagglutinin were crystallized as complexes with the hemagglutinin. The complexes crystallize in PEG 600, pH 6.0, and PEG 2000, pH 8.5, respectively. Both crystals belong to space group P321, with very similar unit cell dimensions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / chemistry*
Antibodies, Monoclonal / isolation & purification
Antibodies, Monoclonal / metabolism
Bromelains / chemistry
Bromelains / metabolism
Crystallization
Crystallography, X-Ray*
Electrophoresis, Polyacrylamide Gel
Hemagglutinins, Viral / chemistry*
Hemagglutinins, Viral / immunology
Hemagglutinins, Viral / metabolism*
Immunoglobulin Fab Fragments / chemistry*
Immunoglobulin Fab Fragments / metabolism
Orthomyxoviridae / chemistry*

Substances

Antibodies, Monoclonal
Hemagglutinins, Viral
Immunoglobulin Fab Fragments
Bromelains