Fractions of trisulfated trihydroxysteroids from the sponges Trachyopsis halichondroides (two different collections) and Cymbastela coralliophila have been isolated and investigated. Ten triacetates were obtained from these fractions by desulfatation/acetylation followed by chromatographic separation, and their structures have been established. Four of these derivatives--namely, triacetates of 5 alpha-pregnane-2 beta,3 alpha,6 alpha-triol (3c), 23,24-dinor-5 alpha-cholane-2 beta,3 alpha,6 alpha-triol (4c), 5 alpha-cholest-22-ene-2 beta,3 alpha,6 alpha-triol (7c), and 24-isopropyl-5 alpha-cholestane-2 beta,3 alpha,6 alpha-triol (11c)-- are described as new derivatives of natural steroids.