Abstract
A monoclonal antibody directed against the 8 kDa subunit of antigen B of Echinococcus granulosus was raised. This antibody was used for purification of antigen B by affinity chromatography. SDS-PAGE and immunological analysis of the purified antigen B demonstrated that the 8 kDa antigen B subunit was purified to homogeneity. The purified antigen retained its strong immunoreactivity in ELISA using human hydatid sera. Furthermore, a sandwich ELISA was established for detection of antigen B from hydatid cysts. The usefulness of this test system was demonstrated by the detection of antigen B in human hydatid cyst fluids, thus confirming the diagnosis of an echinococcal disease.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Helminth*
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Antibodies, Monoclonal*
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Antigens, Helminth / analysis*
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Antigens, Helminth / immunology
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Antigens, Helminth / isolation & purification
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Echinococcosis / diagnosis*
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Echinococcosis / parasitology
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Echinococcosis / veterinary
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Echinococcus / immunology*
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Echinococcus / isolation & purification
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Enzyme-Linked Immunosorbent Assay / methods
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Helminth Proteins*
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Humans
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Lipoproteins / analysis*
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Lipoproteins / immunology
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Lipoproteins / isolation & purification
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Mice
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Mice, Inbred BALB C
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Sheep
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Sheep Diseases / diagnosis
Substances
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Antibodies, Helminth
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Antibodies, Monoclonal
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Antigens, Helminth
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Helminth Proteins
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Lipoproteins
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antigen B, Echinococcus