Cloning and expression of a murine cDNA homologous to the human RCK/P54, a lymphoma-linked chromosomal translocation junction gene on 11q23

Gene. 1995 Dec 12;166(2):293-6. doi: 10.1016/0378-1119(95)00559-5.

Abstract

The RCK/P54 gene was previously isolated from the chromosome translocation breakpoint region on 11q23 of a human lymphoma cell line, RC-K8, with t(11;14)(q23;q32). It was found to encode a 472-483-amino-acid (aa) polypeptide belonging to an RNA helicase/translation initiation factor family. The aim of the present investigation was the isolation and comparative sequence analysis of the mouse RCK/P54 cDNA from a BALB/c spleen cDNA library. Sequence analysis revealed an open reading frame (ORF) predicting a polypeptide of 483 aa showing 97.7% identity to human RCK/p54. Northern analysis demonstrated a 7.5-kb signal in all tissues tested and in vitro translation analysis showed a 54-kDa polypeptide. These results indicate that both mouse and human RCK/P54 are highly conserved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chromosomes, Human, Pair 11
  • Cloning, Molecular
  • DEAD-box RNA Helicases
  • DNA Helicases / chemistry
  • DNA, Complementary
  • Gene Expression
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Peptide Initiation Factors / chemistry
  • Proto-Oncogene Proteins / genetics*
  • RNA Nucleotidyltransferases*
  • RNA, Messenger / genetics
  • Recombinant Proteins
  • Sequence Homology, Amino Acid
  • Translocation, Genetic

Substances

  • DNA, Complementary
  • Peptide Initiation Factors
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Recombinant Proteins
  • RNA Nucleotidyltransferases
  • Ddx6 protein, mouse
  • DDX6 protein, human
  • DNA Helicases
  • DEAD-box RNA Helicases

Associated data

  • GENBANK/D50494