CD34+ cell immunoselection from G-CSF-alone-primed peripheral blood in children with low body mass

Br J Haematol. 1995 Oct;91(2):431-3. doi: 10.1111/j.1365-2141.1995.tb05318.x.

Abstract

We report the data of CD34+ cell immunoselection from peripheral blood after G-CSF-alone mobilization (10 micrograms/kg/d s.c.) in nine children with neuroblastoma (median age 4-5 years (2-8), median body weight 16 kg (10-20). Leukaphereses were carried out on a Cobe Spectra separator and two consecutive harvests (4 blood volumes processed) were used for immunoselection on a Ceprate column. The yield of CD34+ cells in the purified fraction was 50% (23-80), with a median number of 2.8 x 10(6) CD34+ cells/kg (1-9.4). All patients were reinfused with selected CD34+ cells after busulfan 600 mg/m2 +melphalan 180 mg/m2 and achieved successful haemopoietic recovery.

MeSH terms

  • Antigens, CD34 / immunology*
  • Body Weight*
  • Busulfan / therapeutic use
  • Child
  • Child, Preschool
  • Granulocyte Colony-Stimulating Factor / pharmacology*
  • Hematopoietic Stem Cell Transplantation
  • Hematopoietic Stem Cells / immunology
  • Humans
  • Leukapheresis
  • Melphalan / therapeutic use
  • Neuroblastoma / drug therapy
  • Neuroblastoma / immunology*

Substances

  • Antigens, CD34
  • Granulocyte Colony-Stimulating Factor
  • Busulfan
  • Melphalan