Abstract
Mutations in the Drosophila genes pimples and three rows result in a defect of sister chromatid separation during mitosis. As a consequence, cytokinesis is also defective. However, cell cycle progression including the mitotic degradation of cyclins A and B is not blocked by the failure of sister chromatid separation, and as a result, metaphase chromosomes with twice the normal number of chromosome arms still connected in the centromeric region are observed in the following mitosis, pimples encodes a novel protein that is rapidly degraded in mitosis. Our observations suggest that Pimples and Three rows act during mitosis to release the cohesion between sister centromeres.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Anaphase / genetics*
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Animals
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Antigens, Protozoan*
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Base Sequence
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Blotting, Northern
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Cell Cycle Proteins / genetics*
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Cell Cycle Proteins / metabolism
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Cell Division / genetics
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Centromere / genetics
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Centromere / metabolism
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Chromatids / genetics*
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Chromosomes / genetics
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Chromosomes / metabolism
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Cloning, Molecular
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Cyclins / metabolism
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Drosophila / genetics*
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Gene Expression Regulation, Developmental / genetics
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Genes, Insect / physiology
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In Situ Hybridization, Fluorescence
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Larva / genetics
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Metaphase / genetics*
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Mitosis / genetics
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Molecular Sequence Data
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Mutation / physiology
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Phenotype
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Protozoan Proteins / genetics
Substances
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Antigens, Protozoan
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Cell Cycle Proteins
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Cyclins
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Protozoan Proteins
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antigen PIM, Theileria parva