Rakicidins, new cytotoxic lipopeptides from Micromonospora sp. fermentation, isolation and characterization

K D McBrien; R L Berry; S E Lowe; K M Neddermann; I Bursuker; S Huang; S E Klohr; J E Leet

doi:10.7164/antibiotics.48.1446

Rakicidins, new cytotoxic lipopeptides from Micromonospora sp. fermentation, isolation and characterization

J Antibiot (Tokyo). 1995 Dec;48(12):1446-52. doi: 10.7164/antibiotics.48.1446.

Authors

K D McBrien¹, R L Berry, S E Lowe, K M Neddermann, I Bursuker, S Huang, S E Klohr, J E Leet

Affiliation

¹ Bristol-Myers Squibb Company, Pharmaceutical Research Institute, Wallingford, Connecticut 06492, USA.

PMID: 8557602
DOI: 10.7164/antibiotics.48.1446

Abstract

The new cytotoxic agents rakicidins A and B were isolated from cultured broth of a Micromonospora sp. Spectroscopic and amino acid analysis has shown that rakicidin A is a new cyclic lipopeptide, consisting of 4-amino-penta-2,4-dienoic acid, 3-hydroxy-2,4,16-trimethyl-heptadecanoic acid, sarcosine, and 3-hydroxyasparagine. Rakicidin B differs by one methylene group in the lipid side chain. These compounds exhibited cytotoxicity against the M109 cell line.

MeSH terms

Animals
Antibiotics, Antineoplastic / chemistry
Antibiotics, Antineoplastic / isolation & purification
Antibiotics, Antineoplastic / pharmacology*
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Fermentation
Lipopeptides
Mice
Micromonospora / metabolism*
Molecular Structure
Peptides, Cyclic / biosynthesis
Peptides, Cyclic / chemistry
Peptides, Cyclic / isolation & purification
Peptides, Cyclic / pharmacology
Spectrum Analysis
Tumor Cells, Cultured

Substances

Antibiotics, Antineoplastic
Lipopeptides
Peptides, Cyclic
rakicidins