Tectorial membrane regeneration in acoustically damaged birds: an immunocytochemical technique

Hear Res. 1995 Jun;86(1-2):43-6. doi: 10.1016/0378-5955(95)00051-5.

Abstract

A novel immunocytochemical method was used to determine whether the sound-damaged adult quail ear can repair its tectorial membrane (TM) and to compare the repair in quail to that in chicks. Birds were exposed to an octave band noise with a center frequency of 1.5 kHz at 116 dB SPL for 4 h. The chicks were grouped based on recovery duration (0 and 7 days), while the quail were divided into 0-, 7-, and 14-day recovered groups. At the end of the recovery period, the animals were sacrificed, and their basilar papillae labeled with a TM-specific monoclonal primary antibody solution followed by a diaminobenzidine process. Examinations under a stereoscope revealed that a patch lesion devoid of TM was located on all 0-day recovered papillae. Seven days later, a honeycomb-patterned layer was observed covering the lesion. In 14-day recovered quail ears, the honeycomb layer appeared similar to that seen at 7 days post-exposure. These observations indicated that both chicks and quail were able to repair their TM within 7 days following exposure to intense sound.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acoustic Stimulation / adverse effects*
  • Animals
  • Animals, Newborn
  • Antibodies, Monoclonal
  • Basilar Membrane / pathology
  • Basilar Membrane / physiology
  • Basilar Membrane / ultrastructure
  • Chickens
  • Coturnix / physiology*
  • Hearing Loss, High-Frequency / physiopathology*
  • Immunohistochemistry
  • Microscopy, Electron, Scanning
  • Regeneration*
  • Species Specificity
  • Tectorial Membrane / pathology
  • Tectorial Membrane / physiology*
  • Tectorial Membrane / ultrastructure

Substances

  • Antibodies, Monoclonal