Abstract
The flk-2 tyrosine kinase receptor is expressed on hematopoietic stem cells and on acute leukemias (AML and ALL). We have isolated a rat monoclonal antibody (71E1) that binds to this receptor with a relative affinity of 5 nM. The antibody immunoprecipitates both murine and human forms of flk-2 and can block receptor activation by its cognate ligand. In addition, 71E1 inhibits the in vitro proliferation of the murine leukemic cell line, M1, that expresses high levels of flk-2. These results suggest that 71E1 may have utility as both a reagent for elucidating the biological role of flk-2 in hematopoiesis and as an immunotherapeutic in the treatment of acute leukemias.
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal / chemistry
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Antibodies, Monoclonal / isolation & purification*
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Binding Sites, Antibody*
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Extracellular Space / chemistry
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Extracellular Space / immunology
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Humans
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Leukemia, Myeloid, Acute
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Mice
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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Proto-Oncogene Proteins / chemistry*
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Proto-Oncogene Proteins / immunology*
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Rats
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Rats, Inbred Lew
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Receptor Protein-Tyrosine Kinases / chemistry*
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Receptor Protein-Tyrosine Kinases / immunology*
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Tumor Cells, Cultured
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fms-Like Tyrosine Kinase 3
Substances
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Antibodies, Monoclonal
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Proto-Oncogene Proteins
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FLT3 protein, human
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Flt3 protein, mouse
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Receptor Protein-Tyrosine Kinases
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fms-Like Tyrosine Kinase 3