Expression of cell adhesion molecules in placentae from pregnancies complicated by pre-eclampsia and intrauterine growth retardation

Placenta. 1995 Oct;16(7):579-87. doi: 10.1016/0143-4004(95)90027-6.

Abstract

Platelets and neutrophils are involved in maternal placental vascular damage in pre-eclampsia. Recruitment of these cells is probably mediated by cell adhesion molecules expressed at the uteroplacental bed. It remains controversial as to whether platelets and neutrophils mediate damage to trophoblast or villous vasculature. The purpose of this study was to determine the expression of cell adhesion molecules in placentae from normal pregnancies and pregnancies complicated by pre-eclampsia and intrauterine growth retardation (IUGR). Immunostaining for platelet endothelial cell adhesion molecule (PECAM) and intercellular adhesion molecule-1 (ICAM-1) was localized mainly to the endothelium of stem villi, intermediate villi, terminal villi and decidual vessels. Scattered staining for ICAM-1 was also evident in the stroma and fetal membranes. The endothelium of stem villi, intermediate villi and terminal villi were all negative for vascular cell adhesion molecule-1 (VCAM-1) and E-Selectin. PECAM, ICAM-1 and ICAM-2 mRNA were all detectable in normal placentae using northern blotting analysis whereas mRNA for E-Selectin and VCAM-1 were both undetectable. There were no differences in cell adhesion molecule immunostaining or mRNA expression in placentae from pregnancies complicated by pre-eclampsia and IUGR inconclusion, expression of cell adhesion molecules in placentae from pre-eclampsia and IUGR are consistent with a normal physiological role in vascular function.

Publication types

  • Clinical Trial
  • Controlled Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / analysis
  • Antigens, Differentiation, Myelomonocytic / analysis
  • Blotting, Northern
  • Cell Adhesion Molecules / analysis*
  • Cell Adhesion Molecules / genetics
  • E-Selectin / analysis
  • Female
  • Fetal Growth Retardation / complications
  • Fetal Growth Retardation / metabolism*
  • Humans
  • Immunohistochemistry
  • Intercellular Adhesion Molecule-1 / analysis
  • Placenta / chemistry*
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Pre-Eclampsia / complications
  • Pre-Eclampsia / metabolism*
  • Pregnancy
  • RNA / analysis
  • Vascular Cell Adhesion Molecule-1 / analysis

Substances

  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • Cell Adhesion Molecules
  • E-Selectin
  • ICAM2 protein, human
  • Platelet Endothelial Cell Adhesion Molecule-1
  • Vascular Cell Adhesion Molecule-1
  • Intercellular Adhesion Molecule-1
  • RNA