Abstract
We have used alanine scanning to analyze protein-protein interactions by human TATA-element binding protein (TBP) within the transcription preinitiation complex. The results indicate that TBP interacts with RNA polymerase II and general transcription factors IIA, IIB, and IIF within the functional transcription preinitiation complex and define the determinants of TBP for each of these interactions. The results permit construction of a model for the structure of the preinitiation complex.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviruses, Human / genetics
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DNA, Viral / chemistry
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DNA, Viral / metabolism
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / isolation & purification
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DNA-Binding Proteins / metabolism*
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Eukaryotic Cells
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Humans
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Kinetics
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Models, Molecular
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Nucleic Acid Conformation*
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Peptide Chain Initiation, Translational*
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Promoter Regions, Genetic*
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Protein Binding
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Protein Conformation*
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Protein Structure, Secondary
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RNA Polymerase II / chemistry*
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RNA Polymerase II / isolation & purification
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RNA Polymerase II / metabolism*
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TATA-Box Binding Protein
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Transcription Factor TFIIA
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Transcription Factor TFIIB
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Transcription Factors / chemistry*
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Transcription Factors / isolation & purification
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Transcription Factors / metabolism*
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Transcription Factors, TFII*
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Transcription, Genetic
Substances
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DNA, Viral
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DNA-Binding Proteins
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TATA-Box Binding Protein
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Transcription Factor TFIIA
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Transcription Factor TFIIB
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Transcription Factors
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Transcription Factors, TFII
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RNA Polymerase II
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transcription factor TFIIF