Use of nondenaturing silver-stained polyacrylamide gel analysis of polymerase chain reaction amplification products for the differential diagnosis of Leptospira interrogans infection

M A Oliveira; O L Caballero; E Dias Neto; M C Koury; A J Romanha; J Carvalho; R A Hartskeerl; A J Simpson

doi:10.1016/0732-8893(95)00145-3

Use of nondenaturing silver-stained polyacrylamide gel analysis of polymerase chain reaction amplification products for the differential diagnosis of Leptospira interrogans infection

Diagn Microbiol Infect Dis. 1995 Aug;22(4):343-8. doi: 10.1016/0732-8893(95)00145-3.

Authors

M A Oliveira¹, O L Caballero, E Dias Neto, M C Koury, A J Romanha, J Carvalho, R A Hartskeerl, A J Simpson

Affiliation

¹ Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.

PMID: 8582141
DOI: 10.1016/0732-8893(95)00145-3

Abstract

A 285-bp DNA fragment was amplified using the polymerase chain reaction from 38 Leptospira serovars of six different genomic species. The fragments amplified exhibited differential mobilities on nondenaturing polyacrylamide gels resulting from sequence-dependent conformational alterations. Leptospira interrogans serovars could be distinguished from those of other species on this basis.

MeSH terms

Bacterial Typing Techniques
Base Sequence
DNA, Bacterial / analysis*
Diagnosis, Differential
Electrophoresis, Polyacrylamide Gel
Humans
Leptospira interrogans* / classification
Leptospira interrogans* / genetics
Molecular Sequence Data
Polymerase Chain Reaction*
Sensitivity and Specificity
Silver Staining
Weil Disease / diagnosis*

Substances

DNA, Bacterial

Associated data

GENBANK/S81410