Increased expression of early growth response-1 messenger ribonucleic acid in prostatic adenocarcinoma

J Urol. 1996 Mar;155(3):975-81.

Abstract

Purpose: We isolated genes whose expression differs between normal and malignant prostate.

Materials and methods: Differential display polymerase chain reactions revealed a messenger ribonucleic acid (mRNA) with higher expression in prostatic adenocarcinoma versus age matched normal tissue. Deoxyribonucleic acid sequencing identified this mRNA as the product of the early growth response-1 gene.

Results: Early growth response-1 mRNA levels were elevated in 12 of 12 intraprostatic adenocarcinomas but not in breast or ovarian cancers, or in rapidly dividing rat ventral prostate cells. Early growth response-1 mRNA was detected in epithelial and stromal cells at tumor margins but not in lymph node metastases.

Conclusions: Early growth response-1, a nuclear transcription factor, is implicated in the growth and invasion of intraprostatic cancers.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / chemistry
  • Adenocarcinoma / genetics*
  • Animals
  • Base Sequence
  • DNA-Binding Proteins / genetics*
  • Early Growth Response Protein 1
  • Gene Expression Regulation, Neoplastic*
  • Immediate-Early Proteins*
  • Male
  • Molecular Sequence Data
  • Prostatic Neoplasms / chemistry
  • Prostatic Neoplasms / genetics*
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis*
  • Rats
  • Rats, Sprague-Dawley
  • Transcription Factors / genetics*
  • Zinc Fingers / genetics*

Substances

  • DNA-Binding Proteins
  • Early Growth Response Protein 1
  • Egr1 protein, rat
  • Immediate-Early Proteins
  • RNA, Messenger
  • Transcription Factors