Abstract
Objective:
To characterize the effect of added fibronectin (Fn) on human rheumatoid synoviocytes (RAS).
Methods:
Early passage cultured RAS were studied by fluorescent imaging microscopy with multiple parameter overlaying and confocal laser scanning microscopy.
Results:
Added Fn induced a localized decrease in matrix Fn at sites of cell overlap. Similar matrix depletion could be induced by collagen VI, cell binding (120 k) and heparin binding (60 k) fragments of Fn, but not by gelatin binding fragment (45 k).
Conclusion:
The decrease in matrix Fn was associated with the induction of a transformation-like phenotype of overlapping cell growth. Both phenomena were inhibited by serine protease inhibitors.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arthritis, Rheumatoid / metabolism
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Arthritis, Rheumatoid / pathology*
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Cell Division / physiology
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Cells, Cultured / cytology
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Chymotrypsin / antagonists & inhibitors
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Collagen / drug effects
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Collagen / metabolism
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Culture Media
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Extracellular Matrix / chemistry
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Extracellular Matrix / drug effects*
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Extracellular Matrix / pathology
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Fibroblasts / pathology
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Fibronectins / pharmacology*
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Humans
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Image Processing, Computer-Assisted
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Microscopy, Confocal
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Middle Aged
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Osteoarthritis / pathology
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Peptide Fragments / pharmacology
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Serine Proteinase Inhibitors / pharmacology*
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Synovial Membrane / cytology*
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Synovial Membrane / metabolism
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Time Factors
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Trypsin Inhibitors / pharmacology
Substances
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Culture Media
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Fibronectins
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Peptide Fragments
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Serine Proteinase Inhibitors
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Trypsin Inhibitors
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Collagen
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Chymotrypsin