Abstract
Chromosome 6 has been shown to contain at band q27 a minimal region of deletion associated with epithelial ovarian cancers and AF6, a gene disrupted in acute myeloid leukemia with t(6;11)(q27;q23). Using rapid amplification of cDNA ends by polymerase chain reaction, the breakpoint in AF6 was confirmed and a cDNA clone identified. This clone was used as a probe to screen a chromosome 6 cosmid library, and a single cosmid C-109F0645 was isolated. By fluorescence in situ hybridization, C-109F0465 was found to map distal to the critically deleted region associated with ovarian malignancies. AF6 is therefore distinct from and lies telomeric to this region.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute Disease
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Chromosome Mapping
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Chromosomes, Human, Pair 11 / genetics
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Chromosomes, Human, Pair 11 / ultrastructure
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Chromosomes, Human, Pair 6 / genetics*
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Chromosomes, Human, Pair 6 / ultrastructure
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Cosmids / genetics
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Cystadenocarcinoma, Serous / genetics*
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DNA, Complementary / genetics
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DNA, Neoplasm / genetics
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DNA-Binding Proteins / genetics
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Female
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Genes, Tumor Suppressor*
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Histone-Lysine N-Methyltransferase
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Humans
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In Situ Hybridization, Fluorescence
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Kinesins / genetics*
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Leukemia, Myeloid / genetics
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Myeloid-Lymphoid Leukemia Protein
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Myosins / genetics*
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Oncogene Proteins, Fusion / genetics
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Ovarian Neoplasms / genetics*
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Proto-Oncogenes*
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Sequence Deletion*
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Transcription Factors*
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Translocation, Genetic
Substances
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AFDN protein, human
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DNA, Complementary
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DNA, Neoplasm
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DNA-Binding Proteins
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KMT2A protein, human
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Oncogene Proteins, Fusion
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Transcription Factors
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Myeloid-Lymphoid Leukemia Protein
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Histone-Lysine N-Methyltransferase
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Myosins
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Kinesins