The mosquito, Armigeres subalbatus, is naturally resistant to the filarial worm, Brugia malayi, and microfilariae (mf) penetrating the midgut are killed by melanotic encapsulation reactions in the hemocoel within 48 h following ingestion. This vector-parasite system was used to assess changes in hemolymph tyrosine, tyrosine derivatives, and catecholamine-metabolizing enzyme activities using high pressure liquid chromatography with electrochemical detection (HPLC-ED) during melanotic encapsulation reactions against mf. Tyrosine and dopa were detected in the hemolymph of both control and immune-activated (mf-exposed) mosquitoes, but not dopamine or N-acetyl dopamine (NADA). Tyrosine was significantly increased in immune-activated mosquitoes at 6 and 12 h post blood feeding, but was depleted following intrathoracic inoculation of mf in the absence of a blood meal. Dopa also was elevated in immune-activated mosquitoes at 6, 12, and 24 h post-exposure to mf. There were significant increases in phenol oxidase (PO) and dopa decarboxylase (DDC) activities in immune-activated mosquitoes as compared to controls, and these elevated activities were correlated with changes in tyrosine and dopa levels in the hemolymph. No significant differences in N-acetyl transferase (NAT) and dopachrome conversion enzyme (DCE) activities between control and immune-activated mosquitoes were observed. The possible roles these molecules play in melanotic encapsulation reactions of A. subalbatus against mf are discussed.