Abstract
We have previously isolated cDNA of a novel protein (RBQ-1, HGMW-approved symbol RBBP6) that binds to the retinoblastoma gene product (pRB). Total nucleotide sequence of the cDNA has now been determined. It encoded a protein of 140 kDa that consists of 948 amino acids and contains multiple repeated sequences like SRS, YRE, and VPPP. The region used for pRB binding was identified on a small region near the C-terminus. We have mapped this gene to 16p11.2-p12 using polymerase chain reaction analysis on a human-hamster hybrid cell panel and chromosomal fluorescence in situ hybridization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Carrier Proteins / chemistry
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism*
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Chromosome Mapping / methods
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Chromosomes, Human, Pair 16*
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Cricetinae
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DNA, Complementary / isolation & purification
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DNA-Binding Proteins / chemistry
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism*
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HeLa Cells
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Humans
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Hybrid Cells
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In Situ Hybridization, Fluorescence
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Molecular Sequence Data
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Open Reading Frames
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Protein Biosynthesis
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Repetitive Sequences, Nucleic Acid
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Retinoblastoma Protein / metabolism*
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Ubiquitin-Protein Ligases
Substances
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Carrier Proteins
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DNA, Complementary
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DNA-Binding Proteins
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Retinoblastoma Protein
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RBBP6 protein, human
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Ubiquitin-Protein Ligases