Comparison of protocols for depleting anti-E. coli antibody in immunoblotting of recombinant antigens

G Covini; E K Chan; M Colombo; E M Tan; E M Tan

doi:10.1016/0022-1759(95)00288-x

Comparison of protocols for depleting anti-E. coli antibody in immunoblotting of recombinant antigens

J Immunol Methods. 1996 Mar 28;190(1):143-5. doi: 10.1016/0022-1759(95)00288-x.

Authors

G Covini¹, E K Chan, M Colombo, E M Tan, E M Tan

Affiliation

¹ W. M. Keck Autoimmune Disease Center, Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, CA 92013, USA.

PMID: 8601707
DOI: 10.1016/0022-1759(95)00288-x

Abstract

A common problem in immunoblotting for the detection of specific antibodies to recombinant proteins synthesized in E. coli is the presence of contaminating antibodies to E. coli proteins. Four protocols for the efficient depletion of anti-E. coli antibodies from human sera are provided and their uses are discussed.

MeSH terms

Absorption
Antibodies, Bacterial / immunology*
Blotting, Western / methods*
Escherichia coli / immunology*
Humans
Recombinant Proteins / immunology*

Substances

Antibodies, Bacterial
Recombinant Proteins