Genetic analysis of the effects of re-transformation of transgenic lines of the moss Physcomitrella patens

Mol Gen Genet. 1996 Feb 25;250(3):380-2. doi: 10.1007/BF02174397.

Abstract

Genetic analysis of the progeny of crosses involving strains of the moss Physcomitrella patens obtained by re-transforming a stable transgenic line, indicates that the plasmid used for re-transformation inserts at or near the chromosomal location of the related plasmid used to obtain the original transgenic line. The resulting structure may be subject to gene silencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bryopsida / drug effects
  • Bryopsida / genetics*
  • Crosses, Genetic
  • Drug Resistance / genetics
  • Genes, Plant / genetics
  • Genetic Linkage / genetics
  • Hygromycin B / pharmacology
  • Phenotype
  • Plants, Genetically Modified / genetics*
  • Plasmids / genetics*
  • Recombination, Genetic / genetics
  • Transformation, Genetic*
  • Vitamins / metabolism

Substances

  • Vitamins
  • Hygromycin B