A mammalian histone deacetylase related to the yeast transcriptional regulator Rpd3p

Science. 1996 Apr 19;272(5260):408-11. doi: 10.1126/science.272.5260.408.

Abstract

Trapoxin is a microbially derived cyclotetrapeptide that inhibits histone deacetylation in vivo and causes mammalian cells to arrest in the cell cycle. A trapoxin affinity matrix was used to isolate two nuclear proteins that copurified with histone deacetylase activity. Both proteins were identified by peptide microsequencing, and a complementary DNA encoding the histone deacetylase catalytic subunit (HD1) was cloned from a human Jurkat T cell library. As the predicted protein is very similar to the yeast transcriptional regulator Rpd3p, these results support a role for histone deacetylase as a key regulator of eukaryotic transcription.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anti-Bacterial Agents / metabolism
  • Anti-Bacterial Agents / pharmacology
  • Cattle
  • Cell Cycle / drug effects
  • Cloning, Molecular
  • Enzyme Inhibitors / metabolism
  • Enzyme Inhibitors / pharmacology
  • Fungal Proteins / chemistry
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Gene Expression Regulation*
  • Histone Deacetylase Inhibitors
  • Histone Deacetylases / chemistry
  • Histone Deacetylases / genetics
  • Histone Deacetylases / isolation & purification
  • Histone Deacetylases / metabolism*
  • Humans
  • Hydroxamic Acids / metabolism
  • Hydroxamic Acids / pharmacology
  • Molecular Sequence Data
  • Molecular Weight
  • Peptides*
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • T-Lymphocytes / enzymology
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / isolation & purification
  • Transcription Factors / metabolism*
  • Transcription, Genetic*
  • Tumor Cells, Cultured

Substances

  • Anti-Bacterial Agents
  • Enzyme Inhibitors
  • Fungal Proteins
  • Histone Deacetylase Inhibitors
  • Hydroxamic Acids
  • Peptides
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • trapoxin A
  • trichostatin A
  • RPD3 protein, S cerevisiae
  • Histone Deacetylases

Associated data

  • GENBANK/D31480
  • GENBANK/F07807
  • GENBANK/U50079