The nuclear-encoded 18 kDa (IP) AQDQ subunit of bovine heart complex I is phosphorylated by the mitochondrial cAMP-dependent protein kinase

S Papa; A M Sardanelli; T Cocco; F Speranza; S C Scacco; Z Technikova-Dobrova

doi:10.1016/0014-5793(95)01532-9

The nuclear-encoded 18 kDa (IP) AQDQ subunit of bovine heart complex I is phosphorylated by the mitochondrial cAMP-dependent protein kinase

FEBS Lett. 1996 Feb 5;379(3):299-301. doi: 10.1016/0014-5793(95)01532-9.

Authors

S Papa¹, A M Sardanelli, T Cocco, F Speranza, S C Scacco, Z Technikova-Dobrova

Affiliation

¹ Institute of Medical Biochemistry and Chemistry, CNR, University of Bari, Italy.

PMID: 8603710
DOI: 10.1016/0014-5793(95)01532-9

Abstract

In bovine heart mitochondria a protein of M(r) 18 kDa, phosphorylated by mtPKA, is associated to the NADH-ubiquinone oxidoreductase in the inner membrane and is present in purified preparation of this complex. The 18 kDa phosphoprotein has now been isolated and sequenced. It is identified as the 18 kDa (IP) AQDQ subunit of complex I, a protein of 133 amino acids with a phosphorylation consensus site RVS at position 129-131.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Cattle
Cyclic AMP-Dependent Protein Kinases / metabolism*
Electron Transport Complex I
Mitochondria, Heart / enzymology*
Molecular Sequence Data
NADH, NADPH Oxidoreductases / genetics*
NADH, NADPH Oxidoreductases / metabolism
Phosphorylation
Sequence Alignment

Substances

NADH, NADPH Oxidoreductases
Cyclic AMP-Dependent Protein Kinases
Electron Transport Complex I