Abstract
CArG boxes, whose consensus sequence is CC(A/T)6GG, are involved in two very different types of transcriptional responses: response of immediate early genes to serum, mediated by so-called Serum Response Elements (SRE), and transcriptional activation of muscle-specific genes during muscle differentiation. Although previous studies have shown that the Serum Response Factor (SRF) binds to muscular CArG boxes, the role of such a binding in muscle-specific activation of CArG box-dependent genes was not directly demonstrated. Here, by transient co-transfection experiments, we demonstrate that intact SRF is required for muscle-specific transcriptional activation through CArG boxes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / biosynthesis*
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Animals
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Avian Sarcoma Viruses / genetics
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Base Sequence
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Cell Line
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DNA / genetics
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DNA / metabolism*
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DNA-Binding Proteins / metabolism*
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Genetic Vectors
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Humans
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Kinetics
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Molecular Sequence Data
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Muscles
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Nuclear Proteins / metabolism*
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Organ Specificity
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Plasmids
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Promoter Regions, Genetic
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Repetitive Sequences, Nucleic Acid
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Serum Response Factor
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Substrate Specificity
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Transcription Factors / metabolism
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Transcriptional Activation*
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Transfection
Substances
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Actins
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DNA-Binding Proteins
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Nuclear Proteins
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Recombinant Proteins
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Serum Response Factor
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Transcription Factors
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DNA