Abstract
The preclinical studies presented here demonstrate that treatment of human non-small cell lung carcinoma and bladder carcinoma cells by a recombinant adenovirus vector, AdCMVpRB94, expressing the N-terminal truncated retinoblastoma (RB) protein (pRB94) completely suppressed the tumorigenicity of the treated tumor cells in nude mice. Furthermore, gene therapy of established human RB- and RB+ bladder xenograft cancers in nude mice by AdCMVpRB94 resulted in regression of the treated tumors. Of note, although both the full-length and the truncated forms of the RB protein, when overexpressed in tumor cells via replication-deficient adenovirus vectors, were capable of suppression of tumor growth, the pRB94 was evidently more potent than the full-length RB protein.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenoviruses, Human / genetics*
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Adenoviruses, Human / physiology
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Animals
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Bone Neoplasms / pathology
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Breast Neoplasms / pathology
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Carcinoma, Non-Small-Cell Lung / pathology
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Carcinoma, Transitional Cell / pathology
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Carcinoma, Transitional Cell / therapy*
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DNA Replication
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DNA, Complementary / genetics
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Defective Viruses / genetics*
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Defective Viruses / physiology
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Genes, Retinoblastoma*
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Genetic Therapy*
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Genetic Vectors / genetics*
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Humans
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Lung Neoplasms / pathology
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Mice
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Mice, Nude
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Neoplasm Transplantation
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Osteosarcoma / pathology
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Peptide Fragments / genetics*
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Retinoblastoma Protein / biosynthesis
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Retinoblastoma Protein / chemistry
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Retinoblastoma Protein / genetics*
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Sequence Deletion
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Transplantation, Heterologous
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Tumor Cells, Cultured
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Urinary Bladder Neoplasms / pathology
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Urinary Bladder Neoplasms / therapy*
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Virus Replication
Substances
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DNA, Complementary
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Peptide Fragments
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Retinoblastoma Protein