Abstract
REGA-1 is a glycoprotein localized to sheath cell processes in the developing CNS when NBs are producing progeny and neurons are maturing and extending processes. It is also present on a subset of muscles and on the lumenal surface of the ectoderm in the embryonic appendages when pioneer neurons are growing into the CNS. REGA-1 is associated with the extracellular side of the cell membrane by a glycosyl-phosphatidylinositol linkage. We have identified a cDNA clone encoding REGA-1 using a sequence from purified protein. Sequence analysis defines REGA-1 as a novel member of the immunoglobulin superfamily containing three immunoglobulin domains and one fibronectin type III repeat. Each Ig domain has distinct sequence characteristics that suggest discrete functions. REGA-1 is similar to other Ig superfamily members involved in cell adhesion events and neurite outgrowth.
Publication types
-
Comparative Study
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Base Sequence
-
Chromatography, Affinity
-
Cloning, Molecular
-
Conserved Sequence
-
DNA Primers
-
DNA, Complementary
-
Embryo, Nonmammalian / cytology*
-
Embryo, Nonmammalian / physiology*
-
Fibronectins / chemistry
-
Glycosylphosphatidylinositols / metabolism*
-
Grasshoppers / embryology*
-
Immunoglobulins / biosynthesis
-
Immunoglobulins / chemistry*
-
Immunohistochemistry
-
Insect Proteins*
-
Membrane Glycoproteins / analysis
-
Membrane Glycoproteins / biosynthesis*
-
Membrane Glycoproteins / chemistry*
-
Mice
-
Molecular Sequence Data
-
Nerve Tissue Proteins / analysis
-
Nerve Tissue Proteins / biosynthesis*
-
Nerve Tissue Proteins / chemistry*
-
Organ Specificity
-
Polymerase Chain Reaction
-
Protein Structure, Secondary
-
Restriction Mapping
-
Sequence Homology, Amino Acid
Substances
-
DNA Primers
-
DNA, Complementary
-
Fibronectins
-
Glycosylphosphatidylinositols
-
Immunoglobulins
-
Insect Proteins
-
Membrane Glycoproteins
-
Nerve Tissue Proteins
-
REGA-1 protein, Schistocerca americana