Abstract
Chimeric simian/human immunodeficiency viruses (SHIVs) that express the env genes derived from distinct HIV type 1 (HIV-1) isolates were tested for the ability to infect rhesus macaques following intravaginal inoculation. SHIVs containing either the HIV-1 HXBc2 or the HIV-1 89.6 envelope glycoproteins were capable of replicating in intravenously inoculated rhesus macaques. However, intravaginal inoculation of animals with these two SHIVs resulted in infection only with the SHIV containing the HIV-1 89.6 glycoprotein. Thus, properties conferred by the envelope glycoproteins in the chimeric virus affect the ability of particular SHIVs to initiate a systemic infection following vaginal inoculation. These results provide indirect support for the hypothesis that the selection of specific viral variants occurs in the genital tracts of individuals exposed to HIV by sexual contact.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acquired Immunodeficiency Syndrome / immunology
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Acquired Immunodeficiency Syndrome / transmission*
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Animals
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Antibody Formation
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Cloning, Molecular
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DNA, Viral / biosynthesis
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DNA, Viral / chemistry
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Female
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Gene Products, env / biosynthesis
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Gene Products, env / genetics
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Gene Products, gag / biosynthesis
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Genes, gag
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HIV* / genetics
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HIV* / isolation & purification
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Macaca mulatta
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Mucous Membrane / virology
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Polymerase Chain Reaction
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Reassortant Viruses* / genetics
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Reassortant Viruses* / isolation & purification
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Sequence Homology, Nucleic Acid
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Simian Acquired Immunodeficiency Syndrome / immunology
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Simian Acquired Immunodeficiency Syndrome / transmission*
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Simian Immunodeficiency Virus* / genetics
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Simian Immunodeficiency Virus* / isolation & purification
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Vagina* / virology
Substances
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DNA, Viral
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Gene Products, env
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Gene Products, gag