Brush border enzymes in evaluation of acute small bowel rejection

Scand J Immunol. 1996 May;43(5):566-73. doi: 10.1046/j.1365-3083.1996.d01-71.x.

Abstract

The change in activity of three disaccharidase enzymes (maltase, sucrase and lactase) was determined according to the method of Dahlqvist during acute rejection in non-immunosuppressed piglet small bowel grafts. In addition, two brush border enzymes, lactase and aminopeptidase, were stained with monoclonal antibodies. Diminishing disaccharidase activity was an early event during rejection. Diminution began 2 days before distinct morphological changes were seen in the mucosal biopsies. Evaluation of disaccharidase activity can thus be used as a confirmatory method in detecting rejection. Reduction in immunohistological staining of lactase and aminopeptidase with monoclonal antibodies and changes in mucosal morphology were observed to progress simultaneously.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Biopsy
  • Disaccharidases / metabolism
  • Female
  • Graft Rejection / enzymology*
  • Immunohistochemistry
  • Intestine, Small / anatomy & histology
  • Intestine, Small / pathology
  • Intestine, Small / transplantation*
  • Lactase
  • Microvilli / enzymology*
  • Staining and Labeling
  • Sucrase / metabolism
  • Swine
  • alpha-Glucosidases / metabolism
  • beta-Galactosidase / metabolism

Substances

  • Antibodies, Monoclonal
  • Disaccharidases
  • Lactase
  • alpha-Glucosidases
  • beta-Galactosidase
  • Sucrase