Abstract
We have transfected a plasmid expressing the transcriptional regulator GC Factor (GCF) into cell lines and have found that the GCF: 1 causes a decrease in the levels of insulin-like growth factor I receptor (IGF-IR) mRNA; 2 causes a decrease in the number of IGF-IRs; and 3 represses the activity of the IGF-IR promoter. In addition, we show that the regulation of IGF-IR expression by GCF plays a physiological role in the control of cellular proliferation in vitro.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Base Sequence
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Cell Division / drug effects
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Culture Media, Serum-Free / pharmacology
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Epidermal Growth Factor / pharmacology
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ErbB Receptors / biosynthesis
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ErbB Receptors / genetics
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Fibroblasts / drug effects
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Fibroblasts / metabolism
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Gene Expression Regulation* / drug effects
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Mice
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Molecular Sequence Data
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Polymerase Chain Reaction
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Receptor, IGF Type 1 / biosynthesis*
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Receptor, IGF Type 1 / genetics
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins / biosynthesis
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Repressor Proteins / genetics
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Repressor Proteins / physiology*
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Transcription, Genetic / drug effects
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Transfection
Substances
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Culture Media, Serum-Free
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GCFC2 protein, human
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Recombinant Fusion Proteins
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Repressor Proteins
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Epidermal Growth Factor
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ErbB Receptors
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Receptor, IGF Type 1