Protective activity of a murine monoclonal antibody against acute and chronic experimental infection with type IV group B streptococcus

J Med Microbiol. 1996 Jun;44(6):475-81. doi: 10.1099/00222615-44-6-475.

Abstract

A murine IgM monoclonal antibody (MAb H11) was developed against the type polysaccharide capsular antigen of group B streptococcus (GBS), serotype IV, after intraperitoneal immunisation of BALB/c mice with heat-killed bacteria. MAb H11 reacted in immunodiffusion with the purified polysaccharide in both its sialylated and desialylated form, giving a line of identity, and opsonised type IV GBS strains in an in vitro assay. When administered at the time of intraperitoneal lethal challenge with homologous GBS, or 4 h earlier, MAb H11 protected 90% of the mice. Protection was still observed when MAb H11 was given 4 h after the challenge. This MAb was strongly effective in preventing septic arthritis induced by type IV GBS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Animals
  • Antibodies, Monoclonal / biosynthesis
  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / therapeutic use
  • Arthritis, Infectious / prevention & control
  • Bacterial Capsules / immunology
  • Chronic Disease
  • Female
  • Hybridomas
  • Immunodiffusion
  • Immunoglobulin M / biosynthesis
  • Immunoglobulin M / immunology
  • Immunoglobulin M / therapeutic use
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Phagocytosis
  • Polysaccharides, Bacterial / immunology
  • Streptococcal Infections / prevention & control*
  • Streptococcus agalactiae / immunology*

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin M
  • Polysaccharides, Bacterial