Evidence that two genomic species of Rhizobium are associated with Medicago truncatula

Arch Microbiol. 1996 Apr;165(4):285-8. doi: 10.1007/s002030050328.

Abstract

Seventy-three isolates of rhizobia sampled from root nodules of Medicago truncatula were analyzed by restriction fragment length polymorphism (RFLP) of DNA regions amplified by the polymerase chain reaction (PCR) targeting the symbiotic plasmid (nifD-K, nodD1, and nodD2 genes) and the chromosome (16S rDNA plus intergenic spacer). Two genotypic groups were found, regardless of the DNA region targeted. These two groups were given the status of genomic species based on results of DNA/DNA hybridization.

Publication types

  • Comparative Study

MeSH terms

  • Base Sequence
  • Chromosomes, Bacterial / genetics
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / genetics
  • Genome, Bacterial
  • Genotype
  • Medicago sativa / microbiology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Plasmids / genetics
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Rhizobium / classification*
  • Rhizobium / genetics*
  • Rhizobium / isolation & purification
  • Species Specificity

Substances

  • DNA Primers
  • DNA, Bacterial
  • DNA, Ribosomal